Immunolocalization of progesterone receptors in binucleate trophoblast cells of the buffalo placenta (Bubalus bubalis)

Autor: Carlos Eduardo Ambrósio, Maria Angélica Miglino, Flávia Thomaz Verechia Pereira, Celina Almeida Furlanetto Mançanares, Ana Flávia de Carvalho
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2007
Předmět:
Zdroj: Biotemas, Vol 20, Iss 2, Pp 99-106 (2007)
Druh dokumentu: article
ISSN: 0103-1643
Popis: The binucleate trophoblast cells (CTBs) of the water buffalo placenta (Bubalus bubalis) were studied with emphasis on the presence of progesterone receptor. Placentomal tissues from 27 buffalos (2-10 months of pregnancy) were processed and embedded in paraplast (Paraplast Embedding Media – Paraplast Plus) to locate the progesterone receptors using the immunohistochemistry technique. The immunohistochemical reaction for progesterone receptor through monoclonal antibody PgR Ab2 showed staining of CTBs, caruncular epithelial and estromal cells and blood vessel estromal pericitos present in the placentome throughout the entire gestational period analyzed. These results indicate the production of progesterone with autocrine and paracrine action in the placentome growth, differentiation and functional regulation.
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