High-speed scattering polarimetry for correlative nerve fiber imaging and multi-modal analysis
| Autor: | der Heiden, Franca auf, Axer, Markus, Amunts, Katrin, Menzel, Miriam |
|---|---|
| Rok vydání: | 2024 |
| Předmět: | |
| Druh dokumentu: | Working Paper |
| Popis: | Three-Dimensional Polarized Light Imaging (3D-PLI) and Computational Scattered Light Imaging (ComSLI) map dense nerve fibers in brain sections with micrometer resolution using visible light. 3D-PLI reconstructs single fiber orientations, while ComSLI captures multiple directions per pixel, offering deep insights into brain tissue structure. Here, we introduce the Scattering Polarimeter, a high-speed correlative microscope to leverage the strengths of both methods. Based on a M\"uller polarimeter, it incorporates variable retarders and a large-area light source for direct and oblique illumination, enabling rapid 3D-PLI and ComSLI measurements as well as measuring the M\"uller matrix per pixel. Applied to human and vervet monkey brain sections, the Scattering Polarimeter generates results comparable to state-of-the-art 3D-PLI and ComSLI setups and creates a multi-modal fiber direction map, integrating the robust fiber orientations obtained from 3D-PLI with fiber crossings from ComSLI. Furthermore, we discuss applications of the Scattering Polarimeter for unprecedented correlative and multi-modal brain imaging. Comment: 29 pages, 8 figures, all study data are available on https://doi.org/10.26165/JUELICH-DATA/CUXQYF |
| Databáze: | arXiv |
| Externí odkaz: |
|