Actuation of Cell Sheets in 3D
| Autor: | Endresen, Kirsten, Murali, Aniruddh, Serra, Francesca |
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| Rok vydání: | 2024 |
| Předmět: | |
| Druh dokumentu: | Working Paper |
| Popis: | The alignment of fibers and cells in living tissues affect their mechanical properties and functionality. In this context, one can draw an analogy between living tissues and nematic liquid crystal elastomers. We explore this analogy by growing fibroblasts to high density on 2D patterned substrates and observing the contraction of cell sheets upon detachment from the substrates. When the fibroblast sheets detach, they undergo an anisotropic contraction, with a maximum contraction along the nematic director, like nematic elastomers do during a phase transition. We first quantify this anisotropy using substrates patterned with stripes to induce uniaxial alignment, finding that the cell sheets resemble nematic elastomers with a negative Poisson ratio. Then, we apply design principles used for programming curvature in nematic elastomers to actuate 3D structures in the detached fibroblast layers, demonstrating an application of these principles. This proof of concept shows the ability to control the 3-D shape through 2-D patterning in soft cell layers, leading to promising avenues to program tissues. Comment: 6 pages, 3 figures, supplemental information |
| Databáze: | arXiv |
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