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UAV remote sensing technology has become a key technology in crop breeding, which can achieve high-throughput and non-destructive collection of crop phenotyping data. However, the multidisciplinary nature of breeding has brought technical barriers and efficiency challenges to knowledge mining. Therefore, it is important to develop a smart breeding goal tool to mine cross-domain multimodal data. Based on different pre-trained open-source multimodal large language models (MLLMs) (e.g., Qwen-VL, InternVL, Deepseek-VL), this study used supervised fine-tuning (SFT), retrieval-augmented generation (RAG), and reinforcement learning from human feedback (RLHF) technologies to inject cross-domain knowledge into MLLMs, thereby constructing multiple multimodal large language models for wheat breeding (WBLMs). The above WBLMs were evaluated using the newly created evaluation benchmark in this study. The results showed that the WBLM constructed using SFT, RAG and RLHF technologies and InternVL2-8B has leading performance. Then, subsequent experiments were conducted using the WBLM. Ablation experiments indicated that the combination of SFT, RAG, and RLHF technologies can improve the overall generation performance, enhance the generated quality, balance the timeliness and adaptability of the generated answer, and reduce hallucinations and biases. The WBLM performed best in wheat yield prediction using cross-domain data (remote sensing, phenotyping, weather, germplasm) simultaneously, with R2 and RMSE of 0.821 and 489.254 kg/ha, respectively. Furthermore, the WBLM can generate professional decision support answers for phenotyping estimation, environmental stress assessment, target germplasm screening, cultivation technique recommendation, and seed price query tasks. |
