Single cells can resolve graded stimuli
| Autor: | Kramar, Mirna, Hahn, Lauritz, Walczak, Aleksandra M, Mora, Thierry, Coppey, Mathieu |
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| Rok vydání: | 2024 |
| Předmět: | |
| Druh dokumentu: | Working Paper |
| Popis: | Cells use signalling pathways as windows into the environment to gather information, transduce it into their interior, and use it to drive behaviours. MAPK (ERK) is a highly conserved signalling pathway in eukaryotes, directing multiple fundamental cellular behaviours such as proliferation, migration, and differentiation, making it of few central hubs in the signalling circuitry of cells. Despite this versatility of behaviors, population-level measurements have reported low information content (\textless 1 bit) relayed through the ERK pathway, rendering the population barely able to distinguish the presence or absence of stimuli. Here, we contrast the information transmitted by a single cell and a population of cells. Using a combination of optogenetic experiments, data analysis based on information theory framework, and numerical simulations we quantify the amount of information transduced from the receptor to ERK, from responses to singular, brief and sparse input pulses. We show that single cells are indeed able to resolve between graded stimuli, yielding over 2 bit of information, however showing a large population heterogeneity. Comment: 13 pages, 5 figures, 10 pages of supplemental material |
| Databáze: | arXiv |
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