Bypass mechanism of F$_1$-ATPase for asymmetric enzyme kinetics

Autor:	Nakayama, Yohei, Toyabe, Shoichi
Rok vydání:	2024
Předmět:	Physics - Biological Physics
Druh dokumentu:	Working Paper
Popis:	We discovered novel enzyme kinetics of F$_1$-ATPase, a biomolecular motor that synthesizes and hydrolyzes adenosine triphosphate (ATP), using single-molecule experiments and numerical simulations. The enzyme kinetics of F$_1$-ATPase followed the Michaelis-Menten equation in ATP hydrolysis but deviated from it in ATP synthesis, indicating asymmetric enzyme kinetics between ATP synthesis and hydrolysis. Numerical analysis based on a theoretical model revealed a bypass mechanism underlying asymmetric enzyme kinetics. In particular, we found that the origin of the asymmetric enzyme kinetics lies in the asymmetry of the allosterism, not in the asymmetry of potential shapes. The asymmetric enzyme kinetics may suggest that F$_1$-ATPase is designed to sustain the rate of ATP synthesis while suppressing the futile ATP consumption. Comment: 6 pages, 5 figures + Supplementary Material (2 pages)
Databáze:	arXiv
Externí odkaz:	http://arxiv.org/abs/2402.17326 Zobrazit plný text záznamu View this record from Arxiv