| Autor: |
Nüesch, Mark F., Pietrek, Lisa, Holmstrom, Erik D., Nettels, Daniel, von Roten, Valentin, Kronenberg-Tenga, Rafael, Medalia, Ohad, Hummer, Gerhard, Schuler, Benjamin |
| Rok vydání: |
2023 |
| Předmět: |
|
| Druh dokumentu: |
Working Paper |
| Popis: |
The conformational dynamics of single-stranded nucleic acids are fundamental for nucleic acid folding and function. However, their elementary chain dynamics have been difficult to resolve experimentally. Here we employ a combination of single-molecule F\"orster resonance energy transfer, nanosecond fluorescence correlation spectroscopy, fluorescence lifetime analysis, and nanophotonic enhancement to determine the conformational ensembles and rapid chain dynamics of short single-stranded nucleic acids in solution. To interpret the experimental results in terms of end-to-end distance dynamics, we utilize the hierarchical chain growth approach, simple polymer models, and refinement with Bayesian inference of ensembles to generate structural ensembles that closely align with the experimental data. The resulting chain reconfiguration times are exceedingly rapid, in the 10-ns range. Solvent viscosity-dependent measurements indicate that these dynamics of single-stranded nucleic acids exhibit negligible internal friction and are thus dominated by solvent friction. Our results provide a detailed view of the conformational distributions and rapid dynamics of single-stranded nucleic acids. |
| Databáze: |
arXiv |
| Externí odkaz: |
|
