Resonant multi-dielectric coverslip for enhanced total internal reflection fluorescence microscopy
| Autor: | Toumi, Yousra, Mouttou, Anita, Lemarchand, Fabien, Demesy, Guillaume, Koc, Cihan, Muriaux, Delphine, Moreau, Antonin, Lumeau, Julien, Favard, Cyril, Lereu, Aude L. |
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| Rok vydání: | 2023 |
| Předmět: | |
| Druh dokumentu: | Working Paper |
| DOI: | 10.1109/ICTON59386.2023.10207549 |
| Popis: | Multi-dielectric coated coverslip can be designed to reach large optical field enhancements when working under proper illumination conditions and in total internal reflection. In an objective-based total internal reflection fluorescence microscopy (TIRF-M), we propose to use the resulting large field enhancement supported in such coverslip to improve TIRF-M sensitivity by amplifying the collected fluorescence signal. We present here the optimization, realization and testing in TIRF-M of resonant coverslips designed to take into account the experimental constraints. The latter's are due to the inverted configuration of TIRF-M together with the use of a high numerical aperture objective. The challenge is therefore to find the best coating design compromising between the angular tolerance and the field enhancement. We will report here enhanced-TIRF-M imaging over model samples but the ultimate goal here is to be able to monitor and quantify dynamics of pathogens at the membrane of living cells. Comment: 2023 23rd International Conference on Transparent Optical Networks (ICTON), Jul 2023, Bucharest, Romania |
| Databáze: | arXiv |
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