| Autor: |
Turnbaugh, Carter, Axelrod, Jeremy J., Campbell, Sara L., Dioquino, Jeske Y., Petrov, Petar N., Remis, Jonathan, Schwartz, Osip, Yu, Zanlin, Cheng, Yifan, Glaeser, Robert M., Mueller, Holger |
| Rok vydání: |
2020 |
| Předmět: |
|
| Druh dokumentu: |
Working Paper |
| DOI: |
10.1063/5.0045496 |
| Popis: |
Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry-Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous-wave intensities of over 450 GW/cm$^{2}$, sufficient to produce the optimal 90{\deg} phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection. |
| Databáze: |
arXiv |
| Externí odkaz: |
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