Electrical probing of COVID-19 spike protein receptor binding domain via a graphene field-effect transistor

Autor:	Zhang, Xiaoyan, Qi, Qige, Jing, Qiushi, Ao, Shen, Zhang, Zhihong, Ding, Mingchao, Wu, Muhong, Liu, Kaihui, Wang, Weipeng, Ling, Yunhan, Zhang, Zhengjun, Fu, Wangyang
Rok vydání:	2020
Předmět:	Physics - Applied Physics Condensed Matter - Mesoscale and Nanoscale Physics Condensed Matter - Materials Science
Druh dokumentu:	Working Paper
Popis:	Here, in an effort towards facile and fast screening/diagnosis of novel coronavirus disease 2019 (COVID-19), we combined the unprecedently sensitive graphene field-effect transistor (Gr-FET) with highly selective antibody-antigen interaction to develop a coronavirus immunosensor. The Gr-FET immunosensors can rapidly identify (about 2 mins) and accurately capture the COVID-19 spike protein S1 (which contains a receptor binding domain, RBD) at a limit of detection down to 0.2 pM, in a real-time and label-free manner. Further results ensure that the Gr-FET immunosensors can be promisingly applied to screen for high-affinity antibodies (with binding constant up to 2*10^11 M^-1 against the RBD) at concentrations down to 0.1 pM. Thus, our developed electrical Gr-FET immunosensors provide an appealing alternative to address the early screening/diagnosis as well as the analysis and rational design of neutralizing-antibody locking methods of this ongoing public health crisis. Comment: 2 figures
Databáze:	arXiv
Externí odkaz:	http://arxiv.org/abs/2003.12529 Zobrazit plný text záznamu View this record from Arxiv