Detection of Proteoglycan Loss from Articular Cartilage using Brillouin Microscopy, with Applications to Osteoarthritis
| Autor: | Wu, Pei-Jung, Masouleh, Maryam Imani, Dini, Daniele, Paterson, Carl, Torok, Peter, Overby, Darryl R., Kabakova, Irina V. |
|---|---|
| Rok vydání: | 2018 |
| Předmět: | |
| Druh dokumentu: | Working Paper |
| Popis: | The degeneration of articular cartilage (AC) occurs in osteoarthritis (OA), which is a leading cause of pain and disability in middle-aged and older people. The early disease related changes in cartilage extra-cellular matrix (ECM) start with depletion of proteoglycan(PG), leading to an increase in tissue hydration and permeability. These early compositional changes are small (<10%) and hence difficult to register with conventional non-invasive imaging technologies (magnetic resonance and ultrasound imaging). Here we apply Brillouin microscopy for detecting changes in the mechanical properties and composition of porcine AC. OA-like degradation is mimicked by enzymatic tissue digestion, and we compare Brillouin microscopy measurements against histological staining of PG depletion over varying digestion times and enzyme concentrations. The non-destructive nature of Brillouin imaging technology opens new avenues for creating minimally invasive arthroscopic devices for OA diagnostics and therapeutic monitoring. Comment: 10 pages, 5 figures |
| Databáze: | arXiv |
| Externí odkaz: |
|