Nanomechanical resonance captures pre-melting transition in DNA unravelling
| Autor: | Jiang, Keren, Khan, Faheem, Thomas, Javix, Phani, Arindam, Thundat, Thomas |
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| Rok vydání: | 2018 |
| Předmět: | |
| Druh dokumentu: | Working Paper |
| Popis: | A double-stranded DNA unravels thermally through intermediate denatured bubble segments. Intrinsically, fluctuations ensue at the bubble boundaries from non-equilibrium (NE) energy exchanges with the environment. However, such details gets obscured by large population kinetics at the macroscale, associating equilibrium pathway to the unravelling landscape. In this work, we capture evidence of fluctuation energetics with picoliter samples in a microfluidic cantilever. We exploit nanomechanical resonance to measure the NE energy exchanges through dissipation, revealing a crucial pre-melting transition at T~42C . This signifies that unravelling possibly proceeds via intermediate collapsed-bubble conformations releasing energy, sufficient to unbind bubble ends, assisting further unbinding. Fluctuation theorem explains the observations opening further avenues to investigate fluctuation kinetics in other biological phenomena that also proceed through similar NE energetics. Comment: Manuscript and Supplementary Information |
| Databáze: | arXiv |
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