Analyzing conformational changes in single FRET-labeled A1 parts of archaeal A1AO-ATP synthase

Autor:	Sielaff, Hendrik, Singh, Dhirendra, Grueber, Gerhard, Börsch, Michael
Rok vydání:	2018
Předmět:	Quantitative Biology - Biomolecules
Druh dokumentu:	Working Paper
Popis:	ATP synthases utilize a proton motive force to synthesize ATP. In reverse, these membrane-embedded enzymes can also hydrolyze ATP to pump protons over the membrane. To prevent wasteful ATP hydrolysis, distinct control mechanisms exist for ATP synthases in bacteria, archaea, chloroplasts and mitochondria. Single-molecule F\"orster resonance energy transfer (smFRET) demonstrated that the C-terminus of the rotary subunit epsilon in the Escherichia coli enzyme changes its conformation to block ATP hydrolysis. Previously we investigated the related conformational changes of subunit F of the A1AO-ATP synthase from the archaeon Methanosarcina mazei G\"o1. Here, we analyze the lifetimes of fluorescence donor and acceptor dyes to distinguish between smFRET signals for conformational changes and potential artefacts. Comment: 12 pages, 6 figures
Databáze:	arXiv
Externí odkaz:	http://arxiv.org/abs/1801.04692 Zobrazit plný text záznamu View this record from Arxiv