| Autor: |
Rodriguez, Jose A., Xu, Rui, Chen, Chien-Chun, Huang, Zhifeng, Jiang, Huaidong, Raines, Kevin S., Nam, Daewoong, Chen, Allan L., Pryor, A. J., Wiegart, Lutz, Song, Changyong, Madsen, Anders, Chushkin, Yuriy, Zontone, Federico, Bradley, Peter J., Miao, Jianwei |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
IUCrJ 2 (2015) 575-583 |
| Druh dokumentu: |
Working Paper |
| DOI: |
10.1107/S205225251501235X |
| Popis: |
A structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffraction imaging (cryo-CDI) could bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, we report for the first time 3D cryo-CDI of a whole, frozen-hydrated cell - in this case a Neospora caninum tachyzoite - using 8 keV X-rays. Our 3D reconstruction reveals the surface and internal morphology of the cell, including its complex, polarized sub-cellular architecture with a 3D resolution of ~75-100 nm, which is presently limited by the coherent X-ray flux and detector size. Given the imminent improvement in the coherent X-ray flux at the facilities worldwide, our work forecasts the possibility of routine 3D imaging of frozen-hydrated cells with spatial resolutions in the tens of nanometres. |
| Databáze: |
arXiv |
| Externí odkaz: |
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