Motility fractionation of bacteria by centrifugation

Autor: Maggi, Claudio, Lepore, Alessia, Solari, Jacopo, Rizzo, Alessandro, Di Leonardo, Roberto
Rok vydání: 2013
Předmět:
Zdroj: Soft Matter 9, 10885-10890 (2013)
Druh dokumentu: Working Paper
DOI: 10.1039/C3SM51223A
Popis: Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can be also used to separate swimming cells having different motility. To do this we study self-propelled bacteria under the influence of an external centrifugal field. Using dynamic image correlation spectroscopy we measure the spatially resolved motility of bacteria after centrifugation. A significant gradient in swimming-speeds is observed for increasing centrifugal speeds. Our results can be reproduced by a model that treats bacteria as "hot" colloidal particles having a diffusion coefficient that depends on the swimming speed.
Comment: 7 pages, 5 figures (in press)
Databáze: arXiv