Motility fractionation of bacteria by centrifugation
Autor: | Maggi, Claudio, Lepore, Alessia, Solari, Jacopo, Rizzo, Alessandro, Di Leonardo, Roberto |
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Rok vydání: | 2013 |
Předmět: | |
Zdroj: | Soft Matter 9, 10885-10890 (2013) |
Druh dokumentu: | Working Paper |
DOI: | 10.1039/C3SM51223A |
Popis: | Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can be also used to separate swimming cells having different motility. To do this we study self-propelled bacteria under the influence of an external centrifugal field. Using dynamic image correlation spectroscopy we measure the spatially resolved motility of bacteria after centrifugation. A significant gradient in swimming-speeds is observed for increasing centrifugal speeds. Our results can be reproduced by a model that treats bacteria as "hot" colloidal particles having a diffusion coefficient that depends on the swimming speed. Comment: 7 pages, 5 figures (in press) |
Databáze: | arXiv |
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