Crowding induced entropy-enthalpy compensation in protein association equilibria

Autor:	Kim, Young C., Mittal, Jeetain
Rok vydání:	2012
Předmět:	Condensed Matter - Soft Condensed Matter Condensed Matter - Statistical Mechanics Quantitative Biology - Quantitative Methods
Druh dokumentu:	Working Paper
DOI:	10.1103/PhysRevLett.110.208102
Popis:	A statistical mechanical theory is presented to predict the effects of macromolecular crowding on protein association equilibria, accounting for both excluded volume and attractive interactions between proteins and crowding molecules. Predicted binding free energies are in excellent agreement with simulation data over a wide range of crowder sizes and packing fraction. It is shown that attractive interactions between proteins and crowding agents counteract the stabilizing effects of excluded volume interactions. A critical attraction strength, for which there is no net effect of crowding, is almost independent of the crowder packing fraction. Comment: 5 pages, 5 figures, 2 tables, to appear in Physical Review Letters
Databáze:	arXiv
Externí odkaz:	http://arxiv.org/abs/1209.6379 Zobrazit plný text záznamu View this record from Arxiv