Optimum Drift Velocity for Single Molecule Fluorescence Bursts in Micro/Nano-Fluidic Channels

Autor:	Kish, Lazar L., Kameoka, Jun, Granqvist, Claes G., Kish, Laszlo B.
Rok vydání:	2012
Předmět:	Physics - General Physics Physics - Instrumentation and Detectors
Zdroj:	Applied Physics Letters 101, (2012) 043120
Druh dokumentu:	Working Paper
DOI:	10.1063/1.4739471
Popis:	Photonic burst histograms can be used to identify single protein molecules in micro/nano-fluidic channels provided the width of the histogram is narrow. Photonic shot noise and residence time fluctuations, caused by longitudinal diffusion, are the major sources of the histogram width. This Communication is a sequel to an earlier Letter of ours [L. L. Kish et al., Appl. Phys. Lett. 99, 143121 (2011)] and demonstrates that, for a given diffusion coefficient, an increase of the drift velocity enhances the relative shot noise and decreases the relative residence time fluctuations. This leads to an optimum drift velocity which minimizes the histogram width and maximizes the ability to identify single molecules, which is an important result for applications. Comment: 4 pages
Databáze:	arXiv
Externí odkaz:	http://arxiv.org/abs/1204.3317 Zobrazit plný text záznamu View this record from Arxiv