Effects of electropermeabilization and gene electrotransfert on the lateral mobility of a GPi anchored protein

Autor:	Escoffre, Jean Michel, Hubert, Marie, Teissié, Justin, Rols, Marie Pierre, Favard, Cyril
Rok vydání:	2012
Předmět:	Quantitative Biology - Biomolecules Physics - Biological Physics Quantitative Biology - Cell Behavior
Druh dokumentu:	Working Paper
Popis:	Electropermeabilization is a method by which molecules can be introduced in living cells through the plasma membrane. Here, we show that electropermeabilization affects the lateral mobility of Rae-1, a GPi anchored protein. Our results shows that 10 to 20 % of the membrane surface is occupied by defaults or pores. Gene electrotransfert also affects the lateral mobility of Rae-1. Furthermore, we clearly show that once inserted into the plasma membrane, DNA totaly excludes Rae-1, indicating that DNA molecules are tightly packed together to forms huge aggregates.
Databáze:	arXiv
Externí odkaz:	http://arxiv.org/abs/1202.1452 Zobrazit plný text záznamu View this record from Arxiv