Insights into the mechanisms of electromediated gene delivery and application to the loading of giant vesicles with negatively charged macromolecules

Autor:	Portet, Thomas, Favard, Cyril, Teissié, Justin, Dean, David S., Rols, Marie-Pierre
Rok vydání:	2011
Předmět:	Physics - Biological Physics Condensed Matter - Soft Condensed Matter Quantitative Biology - Subcellular Processes
Druh dokumentu:	Working Paper
Popis:	We present experimental results regarding the electrotransfer of plasmid DNA into phosphatidylcholine giant unilamellar vesicles (GUVs). Our observations indicate that a direct entry is the predominant mechanism of electrotransfer. A quantitative analysis of the DNA concentration increments inside the GUVs is also performed, and we find that our experimental data are very well described by a simple theoretical model in which DNA entry is mostly driven by electrophoresis. Our theoretical framework allows for the prediction of the amount of transfered DNA as a function of the electric field parameters, and thus paves the way towards a novel method for encapsulating with high efficiency not only DNA, but any negatively charged macromolecule into GUVs. Comment: Accepted for publication in Soft Matter
Databáze:	arXiv
Externí odkaz:	http://arxiv.org/abs/1102.0610 Zobrazit plný text záznamu View this record from Arxiv