Structure from Fleeting Illumination of Faint Spinning Objects in Flight with Application to Single Molecules
| Autor: | Fung, Russell, Shneerson, Valentin, Saldin, Dilano K., Ourmazd, Abbas |
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| Rok vydání: | 2008 |
| Předmět: | |
| Druh dokumentu: | Working Paper |
| Popis: | There are many instances when the structure of a weakly-scattering spinning object in flight must be determined to high resolution. Examples range from comets to nanoparticles and single molecules. The latter two instances are the subject of intense current interest. Substantial progress has recently been made in illuminating spinning single particles in flight with powerful X-ray bursts to determine their structure with the ultimate goal of determining the structure of single molecules. However, proposals to reconstruct the molecular structure from diffraction "snapshots" of unknown orientation require ~1000x more signal than available from next-generation sources. Using a new approach, we demonstrate the recovery of the structure of a weakly scattering macromolecule at the anticipated next-generation X-ray source intensities. Our work closes a critical gap in determining the structure of single molecules and nanoparticles by X-ray methods, and opens the way to reconstructing the structure of spinning, or randomly-oriented objects at extremely low signal levels. Other potential applications include low-dose electron microscopy, ultra-low-signal tomography of non-stationary objects without orientational information, and the study of heavenly bodies. Comment: 11 pages, 3 figures |
| Databáze: | arXiv |
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