Reprogramming Human Melanocytes and Melanoma Cells with Yamanaka Factors
| Autor: | YILMAZER, Açelya, TAHERİ, Hadiseh |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Volume: 46, Issue: 1 35-41 Hacettepe Journal of Biology and Chemistry |
| ISSN: | 2687-475X |
| Popis: | The generation of induced pluripotent stem (iPS) cells from somatic cells showed that cell fate could bemanipulated by simply introducing a few transcription factors. The differentiation potential of iPS cells hasuncovered a wide range of potential applications, including disease modeling, drug screening and regenerativetherapy. In this study, we aimed to induce reprogramming of human melanocytes and melanoma cell linesvia Sendai viral vectors encoding Yamanaka factors. Following transfection, pluripotent stem cell colonieshave emerged in the melanocyte cultures, as evident by phase-contrast and fluorescence microscopy images.However, only cell clusters which were not positive for pluripotency markers were obtained in the melanomacell cultures. This proved that there are differences between healthy and cancer cell reprogramming. Basalgene expression of Yamanaka factors, pluripotency markers and tumor-suppressor genes have been identifiedin order to understand the different responses for cell reprogramming in melanocytes and cancer cell lines.Future studies that will allow efficient reprogramming of cancer cells can improve our knowledge about cancerbiology and therapy |
| Databáze: | OpenAIRE |
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