Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)
| Autor: | BAKER, Luma Abdalmunim, HIDAYET, Hazha Jamal, AL-CHALABI, Nahida S. |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Issue: 3 56-71 The Eurasia Proceedings of Science Technology Engineering and Mathematics |
| ISSN: | 2602-3199 |
| Popis: | Theresearch include studying biochemical and genetic molecular study forparaoxonase1 enzyme and identify some biochemical parameters for serum bloodsamples 68 women with breast cancer and follow-up after take them three dosesof chemotherapy and comparative with 50 normal female as control group.Theresult showed for women with breast cancer a significant increase in (TC,MDA,Total protein, U,A , calcium and BMI) when compared with normal women, theresults also showed for women who have taken chemical doses and women withbreast cancer significant decreased in (PON1, GSH) compared with normal women,the results showed for women who have takenthe first dose significant increase in (MDA,TP, Calcium, U.A , BMI),while second dose and the third dose chemotherapy found significant increase in(TP, U.A ,Calcium ) and significant decreased for (PON1). The results includedsequential analysis for PON1 enzyme ,it has shown results for genetic analyzedselected exon (Exon4), where DNA extraction for normal women and breast cancerwomen and taken their doses of chemotherapy and measure DNA concentration andpurity using Nano-Drop Spectrophotometric Analysis where the result show theDNA concentration ranged between (5.2-68.4) ng/ml where concentration mean forwomen with breast cancer (26.97) ng/ml and (17.49) ng/ml for normal women whilethe first dose 24.74 ng/ml , (16.16-9.70)ng/ml for second and third doserespectively . It has also been used PCR technique amplification and test products PCR analysis of sequentialtechnique by using gel electrophoresis where packet appeared clear withoutdeformation and tested PCR product sequencing for exon4 by capillary automatedsequencer and comparing the result with the normal sequence of the oxon.,the results shows for women have taken first dose deletion mutation called(Remove) frame shift mutation clear nitrogen base one lead to change all codonsafter deletion site so that all amino acid change in poly protien chain whichled to the loss of amino acid (Tyr.) . and mutation type of silent mutation third mutation type ofsubstitution mutation (Replace amino acid (Thr.) by amino acid (Ser.). |
| Databáze: | OpenAIRE |
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