Evaluation of Anti-Inflammatory Activity of Metronidazole Treatment On Carrageenan Induced Paw Edema in Mice
| Autor: | KAZKAYASI, Inci, TELLİ, Gökçen |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Volume: 2, Issue: 47 161-175 Fabad Journal of Pharmaceutical Sciences |
| ISSN: | 1300-4182 2651-4648 |
| Popis: | Objective: Metronidazole is a nitroimidazole derivative antibiotic thathas been used against protozoa and anaerobic organisms for a long time.Furthermore, it has been used in non-infectious inflammatory diseasessuch as acne, Crohn’s disease, periorificial dermatitis, rosacea andseborrheic dermatitis recently. However, the studies about this issue arevery few and its mechanism of action is unknown. The aim of our studyis to evaluate the possible anti-inflammatory activity of metronidazole invivo by using the mice- carrageenan-induced paw edema method.Material and Method: Mice were administered a single dose of 2, 20or 200 mg/kg metronidazole via oral gavage. One hour later, 2%carrageenan was injected sub-plantar to the hind paws. The pawthickness of mice was measured just before the carrageenan injectionand at 1, 2, 3, 4, 24 and 48 hours after injection by dial thicknessgauge. For comparison, another group of mice received indomethacin (10mg/kg, orally) used as a reference drug. IL-1β and TNF-α levels in thepaws of mice were measured by the ELISA method. ANOVA (post-hocBonferroni) and Student’s t tests were used for statistical analysis.Results: Metronidazole displayed equi-potent anti-inflammatory activitywith indomethacin in the carrageenan-induced mouse paw edema model.It is shown that less edema occurred at all doses (2, 20 and 200 mg/kg)compared to the control group and no differences were obtained in effectbetween the doses. It was observed that in metronidazole treated groups,paw thickness returned to baseline values 48 hours after carrageenaninjection, unlike the control group. IL-1β and TNF-α levels, which wereincreased with carrageenan injection, were significantly decreased withmetronidazole treatment.Conclusions: In our study, metronidazole was found to be antiinflammatorydue to its effects on relieving edema and reducing proinflammatorycytokines in the paws of carrageenan-induced mice. Theeffectiveness of metronidazole in treating various non-infectious diseasesin recent years may be due to its anti-inflammatory activity. |
| Databáze: | OpenAIRE |
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