Thin Layer Chromatographic Separation and Quantitation of L-Dopa and L-Tyrosine in Mixtures

Autor: GÜNENDİ, Gülendem, PAMUK, Fahrünnisa
Jazyk: turečtina
Rok vydání: 2014
Předmět:
Zdroj: Volume: 23, Issue: 3 269-274
Turkish Journal of Chemistry
ISSN: 1300-0527
1303-6130
Popis: A new TLC separation and quantitation method for L-tyrosine and L-Dopa mixtures was developed. The minimum tyrosine and Dopa quantities which can be measured by this technique are 0.7 and 1.5 m g respectively. The method may be used to measure the kinetic parameters of polyphenoloxidase as well as to trace the enzyme catalyzed conversion of L-tyrosine into L-Dopa. For a set of 20 measurements the maximum difference between any two measurements of spot areas was found to be 3 mm2 (4.5%) for L-Dopa (4 m g) and 2mm2 (5.6%) for L-tyrosine (1m g).
Databáze: OpenAIRE