Thin Layer Chromatographic Separation and Quantitation of L-Dopa and L-Tyrosine in Mixtures
| Autor: | GÜNENDİ, Gülendem, PAMUK, Fahrünnisa |
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| Jazyk: | turečtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Volume: 23, Issue: 3 269-274 Turkish Journal of Chemistry |
| ISSN: | 1300-0527 1303-6130 |
| Popis: | A new TLC separation and quantitation method for L-tyrosine and L-Dopa mixtures was developed. The minimum tyrosine and Dopa quantities which can be measured by this technique are 0.7 and 1.5 m g respectively. The method may be used to measure the kinetic parameters of polyphenoloxidase as well as to trace the enzyme catalyzed conversion of L-tyrosine into L-Dopa. For a set of 20 measurements the maximum difference between any two measurements of spot areas was found to be 3 mm2 (4.5%) for L-Dopa (4 m g) and 2mm2 (5.6%) for L-tyrosine (1m g). |
| Databáze: | OpenAIRE |
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