Identification and virules of Sphaeropsis tip blight (Sphaeropsis sapinea) on Pinus spp. in Istanbul and Bursa parks
| Autor: | ÜNAL, Filiz, KOCA, Ercan, AŞKIN, Ayşe, KURBETLİ, İlker, SARPKAYA, Kamil |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Volume: 31, Issue: 1 17-21 Acta Biologica Turcica |
| ISSN: | 2458-7893 |
| Popis: | Sphaeropsis sapinea tip blight is one ofthe most destructive fungal diseases of pine trees in the world. It causes shoot blight, canker, collar rot, root diseaseand blue stain of Pinus spp. As a result of surveysperformed in the parks, recreation areas and groves in Istanbul and Bursaprovinces in January, June and October 2015, it was observed that the agentespecially causes disease on Pinusspp. Thedisease was observed as dead, brown needles at the tips of branches, shootblight and cankers on pine stems and branches. Twenty eight diseased pine samples were collected from parks, recreationareas and groves. Totally15 Sphaeropsis sapinea isolates wereobtained. Cultural andmorphological characteristics of the isolates were determined using culturesgrown on potato dextrose agar (PDA).Cultures on PDA were initially whitewith profuse aerial mycelia that after one week turned grey to blackish. Identificationof the pathogen was based on morphological characteristics of the conidia and DNA sequence analysis. Genomic DNA wasextracted and the internal transcribed spacer (ITS) region of the rDNA wasamplified using primers ITS1 and ITS 4. Pathogenicity tests were made with twiginoculation method. All isolates formed lesions on twigs. |
| Databáze: | OpenAIRE |
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