| Autor: |
Wagner CA, Mohebbi N, Uhlig U, Giebisch GH, Breton S, Brown D, Geibel JP |
| Rok vydání: |
2011 |
| Zdroj: |
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY |
| DOI: |
10.5167/uzh-52791 |
| Popis: |
Intercalated cells in the collecting duct system express V type H(+) ATPases which participate in acid extrusion bicarbonate secretion and chloride absorption depending on the specific subtype. The activity of H(+) ATPases is regulated by acid base status and several hormones including angiotensin II and aldosterone. Angiotensin II stimulates chloride absorption mediated by pendrin in type B intercalated cells and this process is energized by the activity of H(+) ATPases. Moreover angiotensin II stimulates bicarbonate secretion by the connecting tubule (CNT) and early cortical collecting duct (CCD). In the present study we examined the effect of angiotensin II (10 nM) on H(+) ATPase activity and localization in isolated mouse connecting tubules and cortical collecting ducts. Angiotensin II stimulated Na(+) independent intracellular pH recovery about 2 3 fold and this was abolished by the specific H(+) ATPase inhibitor concanamycin. The effect of angiotensin II was mediated through type 1 angiotensin II receptors (AT(1) receptors) because it could be blocked by saralasin. Stimulation of H(+) ATPase activity required an intact microtubular network it was completely inhibited by colchicine. Immunocytochemistry of isolated CNT/CCDs incubated in vitro with angiotensin II suggests enhanced membrane associated staining of H(+) ATPases in pendrin expressing intercalated cells. In summary angiotensin II stimulates H(+) ATPases in CNT/CCD intercalated cells and may contribute to the regulation of chloride absorption and bicarbonate secretion in this nephron segment. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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