| Autor: |
Neumann F.R, Dion V, Gehlen L., Taddei A, Tsai-Pflugfelder M., Gasser S.M. |
| Rok vydání: |
2012 |
| Zdroj: |
Genes & Development |
| Popis: |
Chromatin in the interphase nucleus moves in a constrained random walk. Despite extensive study the molecular causes of such movement and its impact on DNA based reactions are unclear. Using high precision live fluorescence microscopy in budding yeast we quantified the movement of tagged chromosomal loci to which transcriptional activators or nucleosome remodeling complexes were targeted. We found that local binding of the transcriptional activator VP16 but not of the Gal4 acidic domain enhances chromatin mobility. The increase in movement did not correlate strictly with RNA polymerase II (PolII) elongation but could be phenocopied by targeting the INO80 remodeler to the locus. Enhanced chromatin mobility required Ino80's ATPase activity. Consistently the INO80 dependent remodeling of nucleosomes upon transcriptional activation of the endogenous PHO5 promoter enhanced chromatin movement locally. Finally increased mobility at a double strand break was also shown to depend in part on the INO80 complex. This correlated with increased rates of spontaneous gene conversion. We propose that local chromatin remodeling and nucleosome eviction increase large scale chromatin movements by enhancing the flexibility of the chromatin fiber. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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