| Autor: |
Klein, Steffen, Wimmer, Benedikt H., Winter, Sophie L., Kolovou, Androniki, Laketa, Vibor, Chlanda, Petr |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| DOI: |
10.1101/2020.02.27.966739 |
| Popis: |
Lamellar bodies (LBs) are surfactant rich organelles in alveolar type 2 cells. LBs disassemble into a lipid-protein network that reduces surface tension and facilitates gas exchange at the air-water interface in the alveolar cavity. Current knowledge of LB architecture is predominantly based on electron microscopy studies using disruptive sample preparation methods. We established a post-correlation on-lamella cryo-correlative light and electron microscopy approach for cryo-FIB milled lung cells to structurally characterize and validate LB identity in their unperturbed state using the well-established ABCA3-eGFP marker. In situ cryo-electron tomography revealed that LBs are composed of lipidic structures unique in organelle biology. We report open-ended membrane sheets frequently attached to the limiting membrane of LBs and so far undescribed dome-shaped protein complexes. We propose that LB biogenesis is driven by parallel membrane sheet import and the curvature of the limiting membrane to maximize lipid storage capacity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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