| Autor: |
Ping, Zhi, Chen, Shihong, Zhou, Guangyu, Huang, Xiaoluo, Zhu, Sha Joe, Chai, Chen, Zhang, Haoling, Lee, Henry H., Chiu, Tsan-Yu, Chen, Tai, Yang, Huanming, Xu, Xun, Church, George M., Shen, Yue |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| DOI: |
10.1101/829721 |
| Popis: |
Motivation DNA has been reported as a promising medium of data storage for its remarkable durability and space-efficient storage capacity. Here, we propose a robust DNA-based data storage method based on a new codec algorithm, namely ‘Yin-Yang’. Results Using this strategy, we successfully stored different file formats in a single synthetic DNA oligonucleotide pool. Compared to most well-established DNA-based data storage coding schemes presented to date, this codec system can achieve a variety of user goals (e.g. reduce homopolymer length to 3 or 4 at most, maintain balanced GC content between 40% and 60% and simple secondary structure with the Gibbs free energy above −30 kcal/mol). It also shows enhanced robustness in transcoding of different data structure and practical feasibility. We tested this codec with an end-to-end experiment including encoding, DNA synthesis, sequencing and decoding. Through successful retrieval of 3 files totaling 2.02 Megabits after sequencing and decoding, our strategy exhibits great qualities of achieving high storing capacity per nucleotide (427.1 PB/gram) and high fidelity of data recovery. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
