| Autor: |
Colinas, Maite, Pollier, Jacob, Vaneechoutte, Dries, Malat, Deniz G., Schweizer, Fabian, De Clercq, Rebecca, Guedes, Joana G., Martínez-Cortés, Teresa, Molina Hidalgo, Francisco J., Sottomayor, Mariana, Vandepoele, Klaas, Goossens, Alain |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| DOI: |
10.1101/2020.05.04.075671 |
| Popis: |
Summary Catharanthus roseus produces a diverse range of specialized metabolites of the monoterpenoid indole alkaloid (MIA) class in a heavily branched pathway. Recent great progress in identification of MIA biosynthesis genes revealed that the different pathway branch genes are expressed in a highly cell type- and organ-specific and stress-dependent manner. This implies a complex control by specific transcription factors (TFs), only partly revealed today. We generated and mined a comprehensive compendium of publicly available C. roseus transcriptome data for MIA pathway branch-specific TFs. Functional analysis was performed through extensive comparative gene expression analysis and profiling of over 40 MIA metabolites in the C. roseus flower petal expression system. We identified a MYB TF and additional members of the known BIS and ORCA regulators. Further detailed study of the ORCA TFs suggests subfunctionalization of ORCA paralogs in terms of branch-specific regulation and synergistic activity with the central jasmonate response regulator MYC2. Moreover, we identified specific amino acid residues within the ORCA DNA-binding domains that contribute to this branch-specific regulation. Our results advance our understanding of TF paralog specificity for which, despite the common occurrence of closely related paralogs in many species, comparative studies are scarce. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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