Swimming ghosts of the halophilic archaeon, Haloferax volcanii

Autor: Kinosita, Yoshiaki, Mikami, Nagisa, Li, Zhengqun, Braun, Frank, Quax, Tessa EF., van der Does, Chris, Ishmukhametov, Robert, Albers, Sonja-Verena, Berry, Richard
Jazyk: angličtina
Rok vydání: 2020
DOI: 10.1101/2020.01.08.899351
Popis: Archaea swim driven by a membrane-embedded rotary machine, the archaellum. Current understanding of the archaellar motor complex is limited by the lack of a versatile model system for its experimental study. Here we establish a membrane permeabilized ghost-cell model which enables control and quantification of ATP-coupled archaellar rotation. We find a high nucleotide selectivity for rotation and no cooperativity which is a different characteristic of in vitro studies.
Databáze: OpenAIRE