| Autor: |
Purkayastha, Purboja, Pendyala, Kavya, Saxena, Ayush S., Hakimjavadi, Hesamedin, Chamala, Srikar, Baer, Charles F., Lele, Tanmay P. |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| DOI: |
10.1101/2020.04.26.061911 |
| Popis: |
The physical properties of biomaterials – such as stiffness or nanostructure- are commonly tailored to direct cell functions in diverse applications. While biochemically induced laboratory cellular evolution is well established, no studies have examined whether population of cells can evolve in response to selection pressure imposed by the physical properties of biomaterials. Here we found that genetically variable populations of fibroblasts increased in fitness upon sustained culture on novel soft biomaterial substrates, whereas clonal populations did not. Whole exome-sequencing combined with simulations of experimental evolution revealed that cells evolved by natural selection and not random drift. Novel cell phenotypes were observed on the soft substrate upon evolution, and different replicate lines evolved the same phenotypes through distinct patterns of gene expression. These results suggest that laboratory cellular evolution on biomaterials is a powerful untapped approach that can be used to generate cell populations with novel genetic and phenotypic properties. One Sentence Summary Genetically variable populations of cells evolve upon sustained culture on a soft substrate to produce novel phenotypes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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