| Autor: |
de Montigny, Jean, Krishnamoorthy, Vidhyasankar, Rozenblit, Fernando, Gollisch, Tim, Sernagor, Evelyne |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| DOI: |
10.1101/2019.12.27.888792 |
| Popis: |
Waves of spontaneous activity sweep across the neonatal mouse retinal ganglion cell (RGC) layer, driven by directly interconnected cholinergic starburst amacrine cells (the only known retinal cholinergic cells) from postnatal day (P) 0-10, followed by waves driven by glutamatergic bipolar cells. We found transient clusters of cholinergic RGC-like cells around the optic disc during the period of cholinergic waves. They migrate towards the periphery between P2-9 and then they disappear. Pan-retinal multielectrode array recordings reveal that cholinergic wave origins follow a similar developmental center-to-periphery pattern. Electrical imaging unmasks hotspots of dipole electrical activity occurring in the vicinity of wave origins. We propose that these activity hotspots are sites for wave initiation and are related to the cholinergic cell clusters, reminiscent of activity in transient subplate neurons in the developing cortex, suggesting a universal hyper-excitability mechanism in developing CNS networks during the critical period for brain wiring. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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