| Autor: |
Ciechonska, Marta, Sturrock, Marc, Grob, Alice, Larrouy-Maumus, Gerald, Shahrezaei, Vahid, Isalan, Mark |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| DOI: |
10.1101/693234 |
| Popis: |
Bacteria likely encounter more environmental conditions and stresses than specific hard-wired gene regulation networks could ever evolve to control. One potential solution is that fitness pressures may lead to phenotypic natural selection upon variations in gene expression, as long as the genes are essential for cell survival and growth 1–11 . Such mechanisms of emergent gene expression or “ gene expression according to need ” remain poorly understood. Here we show that an antibiotic resistance gene displays dose-responsive gene upregulation that is proportional to the applied stress (fitness pressure) supplied by antibiotic exposure. Drawing an analogy with Ohm’s law in electricity (V=IR), we propose that fitness pressure acts similarly to voltage (V), output gene expression to current (I), and resistance (R) to the constraints of cellular machinery, thus describing a linear relationship between gene expression and environmental pressure. Mathematical modelling shows that positive feedbacks between growth and gene expression, and stochasticity at the level of both mRNA and protein expression, are necessary and sufficient for this phenomenon. Overall, we find that the efficiency of the survival gene (antibiotic resistance enzymatic activity) and the amount of fitness pressure (antibiotic) tune the magnitude of the apparent emergent regulation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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