| Autor: |
Leydon, Alexander R., Wang, Wei, Juarez-Solis, Samuel, Zemke, Joseph E., Zahler, Mollye L., Zheng, Ning, Nemhauser, Jennifer L. |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| DOI: |
10.1101/2020.03.04.976134 |
| Popis: |
The corepressor TOPLESS (TPL) is recruited to many promoters, yet the mechanisms by which it inhibits expression of genes is poorly understood. Using a synthetic auxin response circuit in Saccharomyces cerevisiae, we identified two regions of Arabidopsis thaliana TPL that could independently function as repression domains: the LIS1 homology domain (LisH) and the CT11-RanBPM (CRA) domain. The CRA repressor domain required direct interaction with the Mediator subunit MED21 for full function. While corepressor multimerization is highly conserved, we found that multimer formation had minimal influence on TPL repression strength in yeast or in plants. Finally, we showed that the LisH domain may have a conserved role in repression in different proteins, as a LisH domain from the human TBL1 protein could replace TPL in synthetic assays. Our work provides insight into the molecular mechanisms of transcriptional repression, while also characterizing short, autonomous repression domains to augment synthetic biology toolkits. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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