| Popis: |
During clathrin-mediated endocytosis in eukaryotes, actin assembly is required to overcome large membrane tension and turgor pressure. However, the molecular mechanisms that enable the actin machinery to adapt to varying membrane tension remain unclear. Here, we used quantitative microscopy to determine that, upon increased membrane tension, the endocytic machinery of fission yeast cells rapidly adapts by assembling larger amounts of actin. We also demonstrate that cells rapidly reduce their membrane tension using three parallel mechanisms. In addition to the mechanical protection of their cell wall, yeast cells’ eisosomes regulate moderate changes in membrane tension on a minute time scale. Meanwhile, a temporary reduction of the number of endocytic events for 2 to 6 minutes allows cells to add large pools of membrane to the plasma membrane. Our study sheds light on the tight connection between membrane tension regulation and endocytosis in yeast, which are likely conserved among eukaryotes. |
