Popis: |
Enriching the micronutrient content of staple crops via biofortification provides a sustainable solution to iron and zinc deficiency in food. Analytical techniques such as colorimetry, atomic absorption spectrophotometry (AAS), inductively coupled plasma optical emission spectroscopy (ICP-OES) and advanced non-destructive estimation methods like X–ray fluorescence (XRF) spectroscopy (EDXRF) are being used with respect to microelement (Fe and Zn) biofortification strategies. The comparative study shows that EDXRF offers an opportune and an economical tool for screening a large number of genotypes or breeding materials in cereal and millet biofortification programs. EDXRF probably replaces ICP-based and colorimetric methods in the large-scale screening steps and selecting a greater number of lines and helps in the quicker identification of the desired genome. In this review an effort has been made to review widely and routinely used analytical techniques, principles, and application features. |