| Autor: |
Nurković, J., Dolićanin, Z., Tutić, I., Hajrović, Š., Mustafić, F., Todorović, V., Kovačević-Filipović, M. |
| Jazyk: |
srbština |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Praxis medica (2013) 42(3):45-50 |
| ISSN: |
0350-8773 |
| Popis: |
Stem cells are non-specialized body cells at a very early stage of development, which under normal conditions in a given tissue can differentiate into different types of functionally specialized mature cells. Mesenchymal stem cells (MSCs) are attractive candidates for clinical use in the reconstruction of damaged tissue, especially as it can be isolated from various sources and reproduced, and their use does not carry any ethical problems. Isolation methods of MSCs from adipose tissue are based on enzymatic degradation of the obtained materials. Terms for cultivation of mesenchymal stem cells are temperature of 37 °C and the partial pressure of CO2 5%. MSCs are cultured in the medium, often in a-MEM medium with 10% or 20% of fetal calf serum. Under these conditions of cultivation adherent cells form colonies in 7-14 days. MSCs are multipotent and able to differentiate in vitro conditions into Mesodermal differentiation, forming osteoblasts, chondrocytes and adipocytes. However, they can be differentiated into cells of ectodermal origin (such as neurons) and cells of endodermal origin (eg, ß-cells of the pancreatic islets of Langerhans and hepatocytes). The Laboratory for stem cell of the Department of Biomedical Sciences at the State University of Novi Pazar conducts research of MSCs originating from human adipose tissue. In collaboration with surgeons of Health Center of Novi Pazar, and respecting the norms of the Ethics Committee of both institutions in the period from 01.07.2011. to 01.07.2012. were obtained 22 samples of human subcutaneous adipose tissue of patients aged 18 to 65 years. 15 samples successfully completed the process of isolation and cultivation, and 8 induces Mesodermal differentiation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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