| Popis: |
Isatin is an important precursor for the synthesis of various pharmacologically active heterocyclic compounds. Its derivatives possess antimicrobial, antiviral, anti-in-flammatory, anticonvulsive, anti-HIV, anticancer activities, etc. In this paper, 5-Chloroisatin-3-hydrazone was synthesized and identified by elemental microanalysis, as well as FTIR and UV/VIS methods. Its antibacterial activity was tested against Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 43895 and Proteus vulgaris ATTC 8427 in different concentrations. In case of Gram-negative bacteria Proteus vulgaris, 5-Chloroisatin-3-hydrazone showed to be the most efficient at all tested concentrations (inhibition zone is 25 mm) at the concentration of 500 μg∙cm-3. The photolytic stability of synthesized 5-Chloroisatin-3-hydrazone was also investigated by using UV-B and UV-C cumulatively irradiation at different intervals of time. With the increase in the energy of the incoming photons, the rate of degradation 5-Cloroisatin-3-hydrazone also increases. This suggests that the stability of the compound depends on the radiation dose, i.e., on the energy of the incoming photons. The results show that photolysis kinetics obeys the equation of the pseudo-first order. The mechanism of photolysis can be suggested as hydrogen abstraction from DMF by the triplet state of the isatin carbonyl group, with the formation of ketyl and DMF radicals. |
