Bacterias endófitas aisladas de cultivo de arroz con actividad promotora de crecimiento
| Autor: | Barboza García , Adrián, Pérez Cordero, Alexander, Chamorro Anaya , Lina |
|---|---|
| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2022 |
| Předmět: |
Colonization
Rendimiento Endophytic bacteria Performance Bacillus thuringiensis Oryza sativa Production of siderophores Bacterias endófitas Bacillus cereus Producción de sideróforos promoción del crecimiento vegetal Phosphate solubilization Fijación de nitrógeno Plant growth promotion Solubilización de fosfato Fixation of nitrogen Colonización |
| Zdroj: | Biotechnology in the Agricultural and Agroindustrial Sector; Vol. 21 No. 1 (2023): January to June; 28-38 Biotecnología en el Sector Agropecuario y Agroindustrial; Vol. 21 Núm. 1 (2023): Enero a Junio; 28-38 |
| ISSN: | 1692-3561 1909-9959 |
| Popis: | Endophytic bacteria have become an alternative to improve crop production and replace agrochemicals which cause serious environmental damage. In this work, the plant growth promoting activity of endophytic bacteria isolated from different tissues of rice varieties of the Montería municipality, department of Córdoba, Colombia, was evaluated in vitro. The population of endophytic bacteria was isolated in R2A agar culture medium, counted and qualitatively evaluated the capacity of nitrogen fixation, phosphate solubilization and siderophore production. Endophytic bacteria with positive activities were identified by sequencing the 16S rRNA gene with 5 sets of oligonucleotides specific to the firmicutes class, beta-proteobacteria; gamma-proteobacteria; alpha-proteobacteria and actinobacteria. We isolated 107 morphotypes of endophytic bacteria with a higher presence in variety 2000 and lower in variety 67; the colonized major tissue corresponded to the root. Of the 22 isolates, 11 showed the ability to solubilize phosphate, 6 to produce siderophores, and 5 to fix nitrogen. The sequencing results proved the identity of Bacillus cereus and B. thuringiensis, which have the ability to promote growth in rice cultivation through phosphate solubilization, nitrogen fixation and siderophore production. En este trabajo se evaluó in vitro la actividad promotora de crecimiento de bacterias endófitas aisladas de diferentes tejidos de variedades de arroz del municipio Montería, departamento de Córdoba, Colombia. La población de bacterias endófitas se aisló en medio de cultivo agar R2A, se realizó conteo y se evaluó cualitativamente la capacidad de fijación de nitrógeno, solubilización de fosfato y producción de sideróforos. Las bacterias endófitas con actividades positivas fueron identificadas mediante secuenciamiento del gen ARNr 16S con 5 juegos de oligonucleótidos específicos para la clase firmicutes, beta-proteobacteria; gamma-proteobacteria; alfa-proteobacteria y actinobacteria. Se aislaron 107 morfotipo de bacterias endófitas con mayor presencia en la variedad 2000 y menor en la variedad 67; el tejido mayor colonizado correspondió a la raíz. De los 22 aislados, 11 mostraron capacidad de solubilizar fosfato, 6 de producir sideróforos, y 5 de fijar nitrógeno. Los resultados del secuenciamiento comprobaron la identidad de Bacillus cereus y B. thuringiensis, los cuales tienen la capacidad de promover el crecimiento en cultivo de arroz mediante la solubilización de fosfato, fijación de nitrógeno y producción de sideróforos. In this work, we evaluated in vitro the growth promoter activity of endophytic bacteria evaluated in vitro. The endophytic bacteria population was isolated in R2A agar culture medium counted and qualitatively assessed nitrogen fixation capacity, phosphate solubilization and siderophores production. Endophytic bacteria with positive activities were identified by sequencing the rRNA 16S gene with 5 sets of oligonucleotides specific to the firmicutes class, beta-proteobacteria; gamma-proteobacteria; alphaproteobacteria and actinobacteria. 107 morphotype of endophytic bacteria were isolated with a greater presence in the 2000 and lower variety in variety 67; the colonized major tissue corresponded to the root. Of the 22 isolates, 11 showed the ability to solubilize phosphate, 6 to produce siderophores, and 5 nitrogen fixing. The sequencing results verified the identity of Bacillus cereus and B. thuringiensis, which have the ability to promote growth in rice cultivation through phosphate solubilization, nitrogen fixation and production of siderophores. |
| Databáze: | OpenAIRE |
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