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The antitoxic and antimutagenic effect of royal jelly was assayed by a standard testing procedure on Drosophila melanogaster. The similarity of metabolic pathways between Drosophila and mammals makes the test widely applicable for detecting the impact of various potential promutagens and antimutagenic effects could be recognized accordingly. The flies were treated with the potent mutagen MMS, alone and combined with royal jelly. The frequency of sterile males and sex linked recessive lethal mutations increased significantly after MMS treatment and decreased after combined treatment. The results strongly indicate that, in addition to its well documented action on development, life-span and reproductive ability, royal jelly has an antimutagenic potential as well. Antigenotoksični i antimutageni efekat pčelinjeg mleča ispitivan je korišćenjem standardne procedure testiranja pomoću Drosophilae melanogaster. Sličnost metaboličkih puteva između Drosophila i sisara čini ovaj test široko primenljivim u otkrivanju uticaja različitih potencijalnih mutagena i promutagena, a analogno tome se može ispitivati i mogući antimutageni potencijal substanci. Mušice odgovarajućeg genotipa tretirane su jakim mutagenom, metil-metan-sulfonatom (MMS), samostalno i u kombinaciji sa mlečom. Učestalost sterilnih mužjaka i polno vezanih recesivnih letalnih mutacija rasla je značajno nakon tretmana sa MMS i opadala nakon kombinovanog tretmana. Dobijeni rezultati značajno ukazuju da uz već potvrđeni uticaj na razviće, dužinu života i reproduktivnu sposobnost, mleč ima i antimutageni potencijal. Projekat ministarstva br. 1527 |
