| Autor: |
Atolani, O., Areh, E. T., Oguntoye, O. S., Zubair, M. F., Fabiyi, O. A., Oyegoke, R. A., Tarigha, D. E., Adamu, N., Adeyemi, O. S., Kambizi, L., Olatunji, G. A. |
| Rok vydání: |
2019 |
| Popis: |
This work investigates the chemical compositions of Polyalthia longifolia Thw. seed oil with the associated antioxidant, anti-inflammatory, anti-parasite and cytotoxicity potentials. The oil of P. longifolia seed obtained by soxhlet extraction was trans-esterified and the fatty acid profile characterized using gas chromatography mass spectrometry (GC-MS). The antioxidant activity was evaluated using DPPH and ABTS assays. The anti-bacterial and anti-fungi properties of the oil were determine on clinical isolates of the organisms using agar diffusion method. The anti-inflammatory activities, cytotoxicity and anti-parasite potential were evaluated using lipooxygenase, mammalian cell and Toxoplasma gondii assays respectively. P. longifolia seed was observed to contain oleic (30.31%), linoleic acid (19.27%) and palmitic acid (15.11%) as the major fatty acids with low proportion of tricosylic acid (6.10) and stearic acid (5.56%). The oil had significant anti-lipooxygenase activity (IC50=0.70 +/- 0.02 mu g/mL) comparable to indomethacin (IC50=0.53 +/- 0.07 mu g/mL). The DPPH (IC50=55.91 +/- 31.18 mu g/mL) and ABTS (IC50=16.89 +/- 15.50 mu g/mL) antioxidant activity of the oil was lower to the ascorbic acid (IC50=0.34 +/- 0.04 and 0.54 +/- 0.04 mu g/mL). The oil also showed activities against all the tested bacteria and fungi. The highest inhibition was recorded against S. aureus (17 +/- 1mm) at concentration 200mg/mL. Further, the oils showed strong potential to restrict growth of Toxoplasma gondii in vitro, but the parasite growth inhibition was mildly abated in the presence of -tocopherol. The seed oil of the underutilized P. longifolia possesses essential fatty acids which could be responsible for the numerous biological potentials which include anti-lipooxygenase, antioxidant, anti-inflammatory, anti-parasite, anti-microbial and cytotoxic activities. The incorporation of the natural oil into pharmaceuticals or cosmetics may enhance antioxidant, anti-inflammatory, antimicrobial and cytotoxicity potential of such products. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
