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In the present study, 2366 strawberry seedling samples were collected from 49 strawberry growers before planting in Sultanhisar and Kosk districts of Aydin province during 2009-2010 and 2010-2011 strawberry growing seasons. A total of 447 isolates of Fusarium were obtained from diseased roots and crowns of the commercial and farmer-produced seedlings. As a result of pathogenicity tests on healthy strawberry stolons and plants, 291 isolates of Fusarium spp. were found to be pathogenic. All pathogenic isolates were identified based on morphological characteristics and molecular methods by amplifying translation elongation factor (TEF) gene in PCR. Incidence of Fusarium spp. in the crown of strawberry seedlings was found to be 2.1% in the 1St year and 1.1% in the 2nd year while it was found to be 11.6% in the 1st year and 4.8% in the 2nd year in the root of the seedlings. When considering the origin of the seedlings, the presence of Fusarium spp. was 18.8% and 21.9% in transplants produced from stolons in previous season by the farmers while it was 5.2 and 5.6% in commercial frigo seedlings. Contamination rate of fresh seedlings examined in the second year was 5.6%. BLAST analysis of DNA sequences amplified from TEF genes of Fusarium isolates indicated that 73% of the isolates were F. oxysporum and 19% were F. proliferatum. At the end of study, 5 F. solani, 2 F. verticillioides, 2 F. acuminatum, 2 F. redolens, 1 F. arthrosporioides, 1 F. avenaceum, and 1 F. lateritium were also determined from strawberry seedlings. |
