| Autor: |
Kamp, Søren, Stenderup, Karin, Rosada, Cecilia, Jemec, Gregor B, Dam, Tomas Norman |
| Jazyk: |
angličtina |
| Rok vydání: |
2008 |
| Předmět: |
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| Zdroj: |
Kamp, S, Stenderup, K, Rosada, C, Jemec, G B & Dam, T N 2008, ' Introducing Stereology as a Tool to Assess the Severity of Psoriasis ', Journal of Investigative Dermatology, vol. 128, no. suppl 1, pp. 17 no 102 . |
| Popis: |
The purpose of this study was to introduce stereology as a novel tool in assessing the severity of psoriasis.Psoriasis is a well described chronic inflammatory skin disease affecting approximately 2% of the Caucasian population. The severity of psoriasis has been assessed by a multitude of clinical and histological tools in recent medical history. However, studies of the efficacy of potential anti-psoriatic drugs have been hampered by the lack of methods that are both objective and quantitative in nature. Stereology is a microscopy tool based on mathematical statistics applied to histological specimens in order to obtain three-dimensional properties from two-dimensional tissue samples.The psoriasis xenograft model used in this trial is accepted as a leading animal model for psoriasis.Psoriatic skin from psoriatic patients was grafted onto severe combined immunodeficient (SCID) mice. Subsequently, the mice were subjected to intraperitoneal injections five times weekly with either ciclosporin A, a well known anti-psoriatic drug, or PBS (vehicle control) for three weeks. Each group consisted of 3 mice and the treatment groups were blinded to the observers. A punch biopsy from the skin donor was used as baseline control.After completion of the treatment, the mice were sacrificed and punch biopsies taken from the center of the graft. Epidermal thickness was measured using stereological principles.In the ciclosporin A treated group, epidermal thickness was reduced from 0.26 (+/-0.03) mm (baseline) to 0.13 (+/-0.02) mm, corresponding to a 49% decrease whereas the epidermal thickness in the PBS group increased to 0.33 (+/-0.02) mm, corresponding to a 26% increase.This study shows that stereology clearly demonstrates reduction in epidermal thickness during anti-psoriatic treatment compared to the negative control and might prove a very valuable tool when assessing the efficiency of future anti-psoriatic drugs in the psoriasis xenograft model. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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