| Autor: |
Ryø, Laura Barrett, Thomsen, Emil Aagaard, Mikkelsen, Jacob Giehm |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Ryø, L B, Thomsen, E A & Mikkelsen, J G 2019, ' Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery ', Methods in Molecular Biology, pp. 93-109 . https://doi.org/10.1007/978-1-4939-9170-9_7 |
| Popis: |
Genetic information transferred by HIV-1-based lentiviral vectors as single-stranded RNA is converted to double-stranded DNA by reverse transcription and subsequently inserted into the genome of recipient cells. Integration into the genome allows stable, long-term expression of genes-of-interest driven by promoter sequences contained within the vector. This technology can be used as a standard method for production of cells stably expressing Cas9 protein and single guide RNA (sgRNA), the key components of the CRISPR genome editing system. Here, we provide a protocol for production and validation of VSV-G-pseudotyped lentiviral vectors for delivery of the CRISPR system and generation of knockout cell lines. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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