Computer-Aided Design and Production of RNA Origami as Protein Scaffolds and Biosensors

Autor:	Vallina, Néstor Sampedro, Geary, Cody, Jepsen, Mette, Andersen, Ebbe Sloth
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	Computer-Aided Design Proteins Nucleic Acid Conformation RNA/chemistry Biosensing Techniques Nanotechnology/methods DNA/chemistry Nanostructures/chemistry
Zdroj:	Vallina, N S, Geary, C, Jepsen, M & Andersen, E S 2023, ' Computer-Aided Design and Production of RNA Origami as Protein Scaffolds and Biosensors ', Methods in molecular biology (Clifton, N.J.), vol. 2639, pp. 51-67 . https://doi.org/10.1007/978-1-0716-3028-0_3
DOI:	10.1007/978-1-0716-3028-0_3
Popis:	RNA nanotechnology is able to take advantage of the modularity of RNA to build a wide variety of structures and functional devices from a common set of structural modules. The RNA origami architecture harnesses the property of RNA to fold as it is being enzymatically synthesized by the RNA polymerase and enables the design of single-stranded devices that integrate multiple structural and functional RNA motifs. Here, we provide detailed procedures on how to design and characterize RNA origami structures. The process is illustrated by two examples: one that forms lattices and another example that acts as biosensors.
Databáze:	OpenAIRE
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