Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin-layer chromatography

Autor:	Larsen, T., Axelsen, J. A., Ravn, H. W.
Jazyk:	angličtina
Rok vydání:	2004
Předmět:	Fungi Soil Organic materials Paraffin oil Ergosterol TLC Extraction
Zdroj:	Larsen, T, Axelsen, J A & Ravn, H W 2004, ' Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin-layer chromatography ', Journal of Chromatography A, vol. 1026, no. 1-2, pp. 301-304 .
Popis:	A new, simplified method for extraction of ergosterol (ergosta-5,7,22-trien-3b-ol) from fungi in soil and litter was developed using presoaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94-100 % depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with presoaking extraction. Ergosterol was detected with thin layer chromatography (TLC) using fluorodensitometry with a quantification limit of 8 ng. Using visual evaluation of images of TLC plates photographed in UV light the quantification limit was 16 ng.
Databáze:	OpenAIRE
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