| Autor: |
Römbke, Jörg, Becker, B. Dark, Th. Moser, N. Halsall, W. Powley, A. Ruf, C. Scholer, E. Smit, P. Wege, N. Zenz, L., Krogh, Paul Henning |
| Jazyk: |
angličtina |
| Rok vydání: |
2007 |
| Zdroj: |
Römbke, J, Becker, B. Dark, Th. Moser, N. Halsall, W. Powley, A. Ruf, C. Scholer, E. Smit, P. Wege, N. Zenz, L & Krogh, P H 2007, Validation report – Results of an International Ring test According to the Draft Guideline: Predatory mite reproduction test in soil ( Hypoaspis (Geolaelaps) aculeifer ) (med bidrag) . OECD website . < http://www.oecd.org/dataoecd/54/10/38974264.pdf > |
| Popis: |
A new Test Guideline has been developed, which is designed to be used for assessing the effects ofchemical substances in soil on the reproductive output of the soil mite species Hypoaspis (Geolaelaps)aculeifer Canestrini (Acari: Laelapidae). H. aculeifer represents an additional trophic level to the speciesfor which guidelines are already available. The main endpoint is the reproduction of the mites withoutdiscrimination and quantification of the different stages of the reproductive cycle. Based on alreadystandardised OECD and ISO test guidelines as well as ideas from literature, the ad-hoc working groupHASTE (Hypoaspis aculeifer Soil Test) prepared a draft guideline using OECD format in 2005. This grouphas about 30 members from different countries, representing authorities, industry (including contractlaboratories) and universities.Using the draft guideline as a starting point, HASTE developed a study protocol which was afterwardsused in an international ring test. Twelve laboratories performed in total 48 tests using two test chemicals(dimethoate and boric acid) and two test designs (NOEC, ECx). Only five tests were not valid. Seven tests(plus further work) were intended to clarify methodological details, in particular to determine the optimaltest duration. Originally, a test duration of 16 days was proposed but finally 14 days were determined to bemore suitable and the results are at least as sensitive. The change of the test duration was the only relevantchange of the test method caused by the ring test experiences.The results determined in the ring test proved the suitability of the proposed test method. The LC50 valuesfor both test chemicals differed by less than a factor of 2.5 from the mean and no statistically significantdifferences were found between laboratories.The EC50 values from the dimethoate tests differed by lessthan a factor of two from the mean and no statistically significant differences were found betweenlaboratories. In the case of boric acid the range of EC50 values was broader (they differed by less than afactor of five from the mean), but still there were no statistically significant differences betweenlaboratories. Smaller treatment effects could be detected in the tests with higher numbers of replicates.After the discussion of the results of the ring test in a HASTE workshop (January 2007), the group decidedto recommend to include the final draft version of this test guideline into the OECD test guideline program.This recommendation is based on the evidence summarised in this report and in particular its Annexes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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