Cultured Human Mast Cells Are Heterogeneous for Expression of the High-Affinity IgE Receptor FcεRI
| Autor: | Hoffmann, Hans Jürgen, Frandsen, Pernille Munk, Christensen, Lars Harder, Schiøtz, Peter Oluf, Dahl, Ronald |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Zdroj: | Hoffmann, H J, Frandsen, P M, Christensen, L H, Schiøtz, P O & Dahl, R 2011, ' Cultured Human Mast Cells Are Heterogeneous for Expression of the High-Affinity IgE Receptor FcεRI ', International Archives of Allergy and Immunology, vol. 157, no. 3, pp. 246-250 . https://doi.org/10.1159/000328756 |
| DOI: | 10.1159/000328756 |
| Popis: | Objective: We determined the density of FcεRI on mast cells cultured from cord (CBMC) and peripheral blood (PBMC) and studied the kinetics of the response through FcεRI. Methods: Mast cells were cultured from CD133+ progenitors from peripheral or cord blood. FcεRI was stabilized by culture with 2 μg/ml IgE. Cells were activated by addition of anti-FcεRI antibody (1 ng/ml-10 μg/ml). Maximal activation, sensitivity, and cooperativity were determined. Results: All cultures were homogeneous for tryptase and metachromasy. All cells expressing FcεRI could be activated by cross-linking FcεRI to upregulate CD63. PBMC bind 203,000 molecules of IgE/cell. Stabilization of FcεRI with IgE doubled the number of CD63+ cells (p = 0.0001) and increased the sensitivity (from 0.083 to 0.013 μg/ml anti-FcεRI) and the slope factor (from 10.8 to 68) of PBMC but not of CBMC. Anti-IgE reversed these effects (p = 0.0002) but did not reduce activation levels below that of cell lines not stabilized with IgE. Conclusion: Baseline expression of FcεRI is independent of anti-IgE. The fraction of PBMC that binds high levels of IgE can be activated through FcεRI. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|