In vitro rumen total gas and methane formation from Northern hemisphere macroalgae

Autor: Thorsteinsson, Mirka Mølgaard, Hansen, H. H., Nørskov, Natalja, Nielsen, Mette Olaf
Přispěvatelé: Strandberg, E., Pinotti, L., Messori, S., Kenny, D., Lee, M., Hocquette, J.F., Cadavez, V.A.P., Millet, S., Evans, R., Veldkamp, T., Pastell, M., Pollott, G.
Jazyk: angličtina
Rok vydání: 2021
Zdroj: Thorsteinsson, M M, Hansen, H H, Nørskov, N & Nielsen, M O 2021, In vitro rumen total gas and methane formation from Northern hemisphere macroalgae . in E Strandberg, L Pinotti, S Messori, D Kenny, M Lee, J F Hocquette, V A P Cadavez, S Millet, R Evans, T Veldkamp, M Pastell & G Pollott (eds), Book of Abstracts of the 72nd Annual Meeting of the European Federation of Animal Science . Wageningen Academic Publishers, EAAP Book of Abstracts, vol. 27, pp. 196-196, 72nd Annual Meeting of the European Federation of Animal Science, Davos, Switzerland, 29/08/2021 . https://doi.org/10.3920/978-90-8686-918-3
Popis: The effects of 18 Northern hemisphere macroalgae on in vitro feed degradability and associated methane formation were determined using the AnkomRF system. Furthermore, effects of ensiling method of Saccharina latissima on antimethanogenic activity were also investigated. After harvest, S. latissima was either frozen directly (unensiled, U) or vacuum packed for ensiling for 28 days using four different additives (A-D). Dried samples of the 18 macroalgae orS. latissima silages (0.1 g) were incubated with maize silage (0.5 g) as a basal feed in buffered rumen fluid for 48 h. Total gas production (TGP) was measured and emitted gas was collected for end-point determination of contents of CH4. Pure maize silage was incubated as a control. The content of halomethanes in the 18 macroalgae was measured using methanol extraction and GC-MS with a detection level of 1 μg/g dry weight. Asparagopsis taxiformis was used as a positive control. Degradable (d) DM, dOM, TGP and methane formation data were analysed by using a mixed procedure of R. The screening study of the 18 macroalgae showed the largest reduction in methane formation when Fucus spiralis, Dictyota dichotoma, Ulva sp., Sargassum muticum and Fucus serratus were added to maize silage. Furthermore, none of the 18 macroalgae contained detectable levels of halomethanes. For the S. latissima silages, TGP after 48 h was significantly lower for silage A and B and higher for U. There were no differences in dDM and dOM among U, the silage types or maize silage. Methane formation was significantly lower for silage A and B, 3.38±1.11 and 3.98±1.10 ml/g DM, respectively, while the formation was greater from pure maize silage (22.2±1.36 ml/g DM). Thus, it can be concluded that ensiled S. latissima significantly decreased TGP and the methane formation in vitro withoutaffecting dDM or dOM of the basal feed. In addition, several of the Northern hemisphere macroalgae had potential to reduce methane formation without containing halomethanes or affecting the degradability of the basal feed negatively.
Databáze: OpenAIRE